Seven MLS NEXT Pro clubs continue to the Second Round in the 2025 U.S. Open Cup. The oldest continuous team sports tournament in the United States features MLS NEXT Pro clubs for the second season.
Be sure to check the rundown below to get up to speed on the remaining MLS NEXT Pro contingent vyfing for USOC glory and how to watch their matches this week!
Tuesday, April 1
Columbus Crew 2 vs. Lexington SC
Tuesday 6 p.m. | YouTube
Lower.com Field
Brent Adu-Gyamfi scored a brace as Crew 2 cruised past NY Renegades in the First Round. Lexington SC enters the Second Round with a similarly dominant performance, taking down Southern Indiana FC 5-0.
Charlotte Independence vs. Carolina Core FC
Tuesday 7:30 p.m. | YouTube
Mecklenburg County Sportsplex
Charlotte Independence scored two goals in extra time to earn a First Round win over the Long Island Rough Riders. Carolina Core FC advanced after a strong attacking outing against Soda City in a 4-2 win.
Inter Miami CF II vs. Miami FC
Tuesday 7:30 p.m. ET | YouTube
Chase Stadium
Inter Miami CF II faces another local rival after beating Miami United FC 4-2 to open the tournament. Miami Academy standout Yuval Cohen scored a brace in the previous round, carried that form into MLS NEXT Pro Matchweek 3, and earned the MLS NEXT Rising Star of the Matchday honor.
AV Alta FC vs. LAFC2
Tuesday 10:30 p.m. ET | YouTube
Lancaster Municipal Stadium
LAFC2 passed the test against Arizona FC in the First Round, winning 7-1. They advance to face USL League One side AV Alta FC, based in Lancaster, California. This will be their first road test of the year after going unbeaten at home through three matches in all competitions.
Wednesday, April 2
Chattanooga FC vs. Chattanooga Red Wolves SC
Wednesday 7 p.m. ET | YouTube
Finley Stadium
All signs are pointing to a special night in the Scenic City when Chattanooga FC squares off with crosstown rival Chattanooga Red Wolves of USL League 1.
CFC is unbeaten in all competitions in 2025 and has emerged as an early heavyweight contender in the Eastern Conference. They'll surely need no extra motivation to maintain that reputation in front of what's looking to be a capacity crowd on Wednesday.
Tacoma Defiance vs. Spokane Velocity
Wednesday 10 p.m. ET | YouTube
Starfire Sports Complex
The Tacoma Defiance are coming off a BYE Matchweek in MLS NEXT Pro action and should be well-rested for visiting Spokane Velocity.
Spokane, currently third in USL League 1, adanced with a 1-0 win over Ballard FC. The Defiance advanced with a 3-1 win over amateur side Washington Athletic Club.
Ventura County FC vs. El Paso Locomotive
Wednesday 10:30 p.m. ET | YouTube
Dignity Health Sports Park
Ventura County FC will hope they can keep the form that saw them put up a 7-0 scoreline to qualify for the second round. They welcome USL Championship side El Paso Locomotive FC, who topped amateur side Harpos FC 5-1 in the opening round.
